Separation and qualitative analysis of metallic ions by thin layer electrophoresis. 1

Cetylpyridinium Chloride Micelle-mediated Mutual Separation of Zinc, Cadmium, and Mercury Ions by Thin-layer Chromatography

Cationic surfactant-mediated systems containing different concentrations of cetylpyridinium chloride have been used as mobile phases in thin-layer chromatographic separation of metal cations on silica layers. The effect of surfactant concentrations below and above the critical micelle concentration (CMC) on the retention behaviour of metal ions was examined. The best TLC system for rapid mutual...

Thin-layer acrylamide gel electrophoresis.

A thin-layer acrylamide gel electrophoresis technique suitable for analysis of serum or urine proteins is described in detail. The method gives sharp differentiation of protein fractions and is particularly suitable for the analysis of biological solutions containing proteins in low concentration.

Low-voltage separation of phosphoamino acids by silica gel thin-layer electrophoresis in a DNA electrophoresis cell.

A major objective in the characterization of protein kinase activity is to determine phosphoamino acid identity in substrate proteins. Several fundamentally different phosphoamino acid analysis (PAA) procedures are available to identify phosphorylated amino acids (2,3). Phosphoamino acids can be released from proteins by partial hydrolysis (4) and identified directly by oneor two-dimensional hi...

Thin-layer electrophoresis of serum lipoproteins.

A method is described for semiquantitative study of serum lipoproteins, with particular reference to the very low density fractions involved in transport of triglycerides. Serum samples were subjected to electrophoresis in thin layers of starch granules; lipids were extracted from starch segments and subjected to thin-layer chromatography; lipid fractions were recovered from the adsorbent and q...

Separation of Cholesterol and Demosterol by Thin-layer Chromatography.